Olivier Johannes Verhoev

DC4

HRIM-MS for improved therapeutic monoclonal antibody-based formats characterisation

Co-supervisor: Oscar Hernandez-Alba

Centre National de la Recherche Scientifique (Strasbourg, France)

Background

My academic journey began in 2015 when I began my Chemistry Bachelor of Science in Rotterdam, The Netherlands. Here, I acquired the foundation of my chemical knowledge. My Bachelor project focused on the characterisation of 3D-printed concrete (6 months, Concr3de, Rotterdam, The Netherlands) and I graduated in 2020.

In 2021, I continued my academic career. Before I started the Master Chemistry (Track Analytical Sciences) in Amsterdam (joint degree University of Amsterdam and Vrije Universiteit Amsterdam), I followed a pre-Master programme at the same universities. During my Master’s degree, I further developed my theoretical knowledge with a focus on separation sciences and mass spectrometry. For my Master project, I was a member of the MSLaserlab of Anouk Rijs (11 months, Vrije Universiteit Amsterdam). Here, I focused on monitoring the aggregation and on the structural characterisation of mutations in α-synuclein peptides, where I gained extensive experience in IM-MS-IRMPD. I acquired my Master’s degree in November 2024 before joining MobiliTraIN in January 2025.

Several aspects piqued my interest in the MobiliTraIN project: the collaboration between academia and industry, the focus on international collaboration, and of course the developments in ion mobility techniques. I expect that MobiliTraIN will have a significant impact on both the academic as well as the industrial/regulatory environment. Personally, I hope to contribute towards the scientific excellence that is expected of an MSCA PhD candidate and to deliver more standardised platforms for biotherapeutics characterisation with ion mobility.

Secondments

University of Manchester (UNIMAN)

Country:  United Kingdom

Duration: 3 months

Purpose: Acquire hands on experience with IMn experiments and variable temperature IM for in depth interpretation of CIU data

Bruker Daltonics GmbH & Co. KG (Bruker)

Country: Germany

Duration: 3 months

Purpose: Perform CIU analysis on TIMS for comparison with TWIMS data

Quality Assistance S.A.

Country: Belgium

Purpose: Analysis of mAb-based formats on orthogonal analytical techniques for higher order structure characterisation